BioChem - DOS

Effect of Inoculation Process on Lycopene Production by Blakeslea trispora in a Stirred-Tank Reactor

Applied biochemistry and biotechnology

Wang, Qiang; Feng, Ling-ran; Luo, Wei; Li, Han-guang; Zhou, Ya; Yu, Xiao-bin

<P>Lycopene biosynthesis by Blakeslea trispora was greatly enhanced in a stirred-tank reactor when a nonsynchronous inoculation process, in which the (+) mating type was inoculated after the (-) mating type has been grown for a certain period of time, was applied. The lycopene concentration with nonsynchronous inoculation in a 24-h inoculation interval was 33 % higher than that with synchronous inoculation. The optimum inoculation ratio was 1:2 (+/-) at the 36 and 48 h inoculum age of mating types (+) and (-), respectively. Fermentation time for the individual strains and mated conditions showed that the (+) mating type grows faster than the (-) mating type. Morphological observation showed that the mycelium ratio of B. trispora (-) in mating culture with nonsynchronous inoculation was higher than that with synchronous inoculation. The results indicated that nonsynchronous inoculation process increased the dominance of B. trispora (-) in joint cultivation and hence stimulated lycopene biosynthesis.</P>

2015

Springer-Verlag

0273-2289

1559-0291

175

2

pp.770-779

10.1007/s12010-014-1327-y

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART71457108