초록
The production of cellulases and xylanases by Penicillium echinulatum in an airlift bioreactor was evaluated. In batch production, we tested media with isolated or associated cellulose and sorbitol. In fed-batch production, we tested cellulose addition at two different times, 30h and 48h. Higher liquid circulation velocities in the downcomer were observed in sorbitol 10gL<SUP>-1</SUP> medium. In batch production, higher FPA (filter paper activity) and endoglucanase activities were obtained with cellulose (7.5gL<SUP>-1</SUP>) and sorbitol (2.5gL<SUP>-1</SUP>), 1.0UmL<SUP>-1</SUP> (120h) and 6.4UmL<SUP>-1</SUP> (100h), respectively. For xylanases, the best production condition was cellulose 10gL<SUP>-1</SUP>, which achieved 5.5UmL<SUP>-1</SUP> in 64h. The fed-batch process was favorable for obtaining xylanases, but not for FPA and endoglucanases, suggesting that in the case of cellulases, the inducer must be added early in the process.