Expression of mitochondrial cytochrome C oxidase chaperone gene (COX20) improves tolerance to weak acid and oxidative stress during yeast fermentation
메타 데이터
바이오화학분류
바이오플라스틱
플라스틱
바이오정밀화학
용매
화학제품
연료
화장품용 기능성소재
계면활성제⁄증점제
의료용 화학소재
식품첨가제
논문
Expression of mitochondrial cytochrome C oxidase chaperone gene (COX20) improves tolerance to weak acid and oxidative stress during yeast fermentation
학술지
PloS one
저자명
Kumar, Vinod; Hart, Andrew J.; Keerthiraju, Ethiraju R.; Waldron, Paul R.; Tucker, Gregory A.; Greetham, Darren
초록
<P><B>Introduction</B></P><P><I>Saccharomyces cerevisiae</I> is the micro-organism of choice for the conversion of fermentable sugars released by the pre-treatment of lignocellulosic material into bioethanol. Pre-treatment of lignocellulosic material releases acetic acid and previous work identified a cytochrome oxidase chaperone gene (<I>COX20</I>) which was significantly up-regulated in yeast cells in the presence of acetic acid.</P><P><B>Results</B></P><P>A Δ<I>cox20</I> strain was sensitive to the presence of acetic acid compared with the background strain. Overexpressing <I>COX20</I> using a tetracycline-regulatable expression vector system in a Δ<I>cox20</I> strain, resulted in tolerance to the presence of acetic acid and tolerance could be ablated with addition of tetracycline. Assays also revealed that overexpression improved tolerance to the presence of hydrogen peroxide-induced oxidative stress.</P><P><B>Conclusion</B></P><P>This is a study which has utilised tetracycline-regulated protein expression in a fermentation system, which was characterised by improved (or enhanced) tolerance to acetic acid and oxidative stress.</P>