Corn stover hydrolysate (CSH) and cane molasses were studied for butanol fermentation by Clostridium saccharobutylicum DSM 13864 in continuous fermentation. Using cane molasses as substrate, solvent of 13.75g/L (butanol 8.65g/L) and productivity of 0.439g/L/h were achieved in a four-stage continuous fermentation at a gradient dilution mode of 0.15-0.15-0.125-0.1h<SUP>-1</SUP>. In continuous fermentation using CSH as substrate, total solvent titer of 11.43g/L (butanol 7.81g/L) and productivity of 0.429g/L/h were reached at a dilution rate of 0.15h<SUP>-1</SUP>, and the steady process was continuously operated for 220h without compromise in solvent titer.