초록
<P><B>Abstract</B></P> <P>A wide range of biofuels and bio-based products can be produced from lignocellulose considering its high compositional diversity. Ethanol production by yeasts from cellulosic glucose is well-known, while hemicellulosic xylose utilization is still challenging. This work proposes the use the xylose for <SMALL>L</SMALL>-lactic acid fermentation. In this context, a sequential cultivation of <I>Saccharomyces cerevisiae</I> and the C5-utilizing <I>Bacillus coagulans</I> is studied. High ethanol yields, around 0.44 g g<SUP>−1</SUP>, were obtained from a cellulosic-gardening hydrolysate. The high ethanol concentrations did not affect the evolved <I>B. coagulans</I> A20-EXA obtained by adaptive evolution to ethanol. As a result, 2.6-fold increase in lactic acid yield was achieved when compared with parental <I>B. coagulans</I> strain in presence of 5% (v v<SUP>−1</SUP>) ethanol. These results demonstrated the suitability of <I>B. coagulans</I> A20-EXA to be used together with <I>S. cerevisiae</I> for the sequential co-generation of ethanol and lactic acid from lignocellulosic biomass in a biorefinery approach.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>Bacillus coagulans</I> A20-EXA was obtained by adaptive evolution to ethanol. </LI> <LI> 0.44 g g<SUP>−1</SUP> of ethanol yield was obtained from cellulosic gardening hydrolysate. </LI> <LI> Lactic acid yield increased 2.4-fold from xylose in MRS with 6% (v v<SUP>−1</SUP>) ethanol. </LI> <LI> 0.89 g g<SUP>−1</SUP> of lactic acid were yielded from ethanol-rich real and defined media. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>