초록
To overcome induced fatty acid inhibition during dark-fermentative hydrogen (H<SUB>2</SUB>) production process, a hybrid strategy was designed and evaluated by co-culturing photosynthetic bacteria with acidogenic microflora. Augmented dark-photo fermentation system (ADPFS) illustrated 40% increment in cumulative H<SUB>2</SUB> production (CHP, 250 ml) compared to dark-fermentation system (DFS) along with 10% enhancement in COD removal efficiency. Co-culturing helped to reduce VFA accumulation by 40% which supports the functional role of photosynthetic organisms in reducing the fatty acid concentration in association to additional H<SUB>2</SUB> production. Relatively higher reduction in individual fatty acids viz., acetic acid (43%), butyric acid (57%) and propionic acid (65%) was observed with AD-PFS operation. Increment in bacteriochlorophyll (Bchl) after augmentation corroborated well with results. At lower pH, pheophytinization was observed which hindered H<SUB>2</SUB> production. Voltammograms illustrated dominant oxidation behavior during hybrid AD-PFS operation and provides viable option for enhancing performance by regulating system buffering microenvironment.