Metabolic engineering of oleaginous fungus Mortierella alpina for high production of oleic and linoleic acids

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바이오화학분류

- 바이오정밀화학
  1. 화학제품
- 화장품용 기능성소재
  1. 기능성
  2. 계면활성제⁄증점제
- 의료용 화학소재
  1. 식품첨가제

논문

Metabolic engineering of oleaginous fungus Mortierella alpina for high production of oleic and linoleic acids

학술지

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

저자명

Sakamoto, Takaiku; Sakuradani, Eiji; Okuda, Tomoyo; Kikukawa, Hiroshi; Ando, Akinori; Kishino, Shigenobu; Izumi, Yoshihiro; Bamba, Takeshi; Shima, Jun; Ogawa, Jun

초록

Abstract The aim of this work was to study the molecular breeding of oleaginous filamentous Mortierella alpina for high production of linoleic (LA) or oleic acid (OA). Heterologous expression of the Δ12-desaturase (DS) gene derived from Coprinopsis cinerea in the Δ6DS activity-defective mutant of M. alpina increased the LA production rate as to total fatty acid to 5 times that in the wild strain. By suppressing the endogenous Δ6I gene expression by RNAi in the Δ12DS activity-defective mutant of M. alpina, the OA accumulation rate as to total fatty acid reached 68.0%. The production of LA and OA in these transformants reached 1.44 and 2.76g/L, respectively, on the 5th day. The Δ6I transcriptional levels of the RNAi-treated strains were suppressed to 1/10th that in the parent strain. The amount of Δ6II RNA in the Δ6I RNAi-treated strain increased to 8 times that in the wild strain. Highlights <UL> <LI> LA or OA high-producing strains were obtained by molecular breeding of M. alpina. </LI> <LI> The production of LA and OA reached 3.32 and 3.85g/L, respectively, on the 5th day. </LI> <LI> The Δ6II RNA level in the transformant was 8 times as high as the wild strain. </LI> </UL>

발행연도

2017

발행기관

Elsevier

라이선스

publisher-specific-oa

ISSN

0960-8524

권

245

호

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