초록
Nitrogen source optimization combined with phased exponential l-tyrosine feeding was employed to enhance l-phenylalanine production by a tyrosine-auxotroph strain, Escherichia coli YP1617. The absence of (NH<SUB>4</SUB>)<SUB>2</SUB>SO<SUB>4</SUB>, the use of corn steep powder and yeast extract as composite organic nitrogen source were more suitable for cell growth and l-phenylalanine production. Moreover, the optimal initial l-tyrosine level was 0.3 g L<SUP>-1</SUP> and exponential l-tyrosine feeding slightly improved l-phenylalanine production. Nerveless, l-phenylalanine production was greatly enhanced by a strategy of phased exponential l-tyrosine feeding, where exponential feeding was started at the set specific growth rate of 0.08, 0.05, and 0.02 h<SUP>-1</SUP> after 12, 32, and 52 h, respectively. Compared with exponential l-tyrosine feeding at the set specific growth rate of 0.08 h<SUP>-1</SUP>, the developed strategy obtained a 15.33% increase in l-phenylalanine production (l-phenylalanine of 56.20 g L<SUP>-1</SUP>) and a 45.28% decrease in l-tyrosine supplementation.