BioChem - DOS

The family II carbohydrate-binding module of xylanase CflXyn11A from Cellulomonas flavigena increases the synergy with cellulase TrCel7B from Trichoderma reesei during the hydrolysis of sugar cane bagasse

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Pavó n-Orozco, Patricia; Santiago-Herná ndez, Alejandro; Rosengren, Anna; Hidalgo-Lara, Marí a Eugenia; Stå lbrand, Henrik

<P><B>Highlights</B></P><P>&#x025BA; High synergy observed between a cellulase and a hemicellulase (xylanase) during enzymatic hydrolysis of sugar cane bagasse. &#x025BA; The synergy was dependent on time of incubation and molar ratio of the two enzymes. &#x025BA; It was shown that a non-catalytic carbohydrate-binding module plays an important role for the synergy.</P> <P><B>Abstract</B></P><P>Synergy between <I>Cellulomonas flavigena</I> xylanase CflXyn11A and <I>Trichoderma reesei</I> endoglucanase TrCel7B was assessed during hydrolysis of alkaline pretreated sugar cane bagasse (SCB) after 12&ndash;48h, applying the individual enzymes and mixtures of the enzymes. A high degree of synergy (6.3) between CflXyn11A and TrCel7B in hydrolysis of SCB was observed after 12h in the equimolar mixture. A threefold decrease in the degree of synergy was observed with TrCel7B and the catalytic module of CflXyn11A; suggesting an important role played by the carbohydrate-binding module of CflXyn11A (CflXyn11A-CBM) in the observed synergy. Affinity electrophoresis and binding assays showed that CflXyn11A-CBM binds to xylans and to a lesser extent to cellulose. Our results suggest that synergy is more pronounced at early stages of hydrolysis. Furthermore, for the first time it is described that a CBM carried by a xylanase significantly enhances the synergy with a cellulase (threefold increase in synergy).</P>

2012

0960-8524

104

pp.622-630

10.1016/j.biortech.2011.11.068

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART58196847

Cellulase; Xylanase; Synergy; Sugar cane bagasse