초록
<P>Biofuel from lignocellulosic materials is a promising type of fuel because it does not compete with food supplies and has a sustainable production process. However, the primary obstacle for producing biofuels from lignocellulose is the low-energy productivity of microorganisms. In this research, thermophilic bacterial strain DD32, which effectively converts lignocellulose to hydrogen, was isolated and characterized. This strain was identified as<I>Thermoanaerobacterium thermosaccharolyticum</I>according to 16S rRNA sequence homology. The maximum H2production yield reached 12.08 mmol H2g<SUP>−1</SUP>Avicel, which is equivalent to 2.17 mol H2mol<SUP>−1</SUP>glucose, at the optimal condition of 55 °C and pH 7.5 with 5.0 g L<SUP>−1</SUP>Avicel. To our knowledge, this result represents the highest H2yield from cellulose for thermophilic bacterial monocultures reported so far. Moreover, the hydrogen productivity of strain DD32 from raw (non-pretreated) lignocellulosic biomass is also tested. Results show that the highest hydrogen yield and lignocelluloses degradation rate reached 6.38 mmol H2g<SUP>−1</SUP>and 44.29% from corn stalk after 72 h of incubation. This yield was almost 2-3 times that of other thermophilic strains. These results suggested that newly isolate<I>T. thermosaccharolyticum</I>DD32 could serve as an effective microbial catalyst for lignocellulosic hydrogen production.</P>