초록
<P>Fermentation of xylanase produced by <I>Trichoderma reesei </I>was conducted in 250mL shake flasks. Several fermentation conditions was investigated that affected production of xylanase by <I>Trichod erma reesei</I>, including inoculum age, inoculum size, medium volume, shear stress, temperature, shaking speed and initial pH. Results showed that the the optimum culture conditions were as follows: inoculum age 45h, inoculum size 10%,medium volume 50mL/250mL, shaking speed 200rpm and initial pH4.0(natural). The maximum xylanase activity under each condition was occurred at 104h and the maximal activities can reach 1425.27U/mL.</P>