BioChem - DOS

Effects of changes in chemical and structural characteristic of ammonia fibre expansion (AFEX) pretreated oil palm empty fruit bunch fibre on enzymatic saccharification and fermentability for biohydrogen

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Abdul, P.M.; Jahim, J.Md.; Harun, S.; Markom, M.; Lutpi, N.A.; Hassan, O.; Balan, V.; Dale, B.E.; Mohd Nor, M.T.

Oil palm empty fruit bunch (OPEFB) fibre is widely available in Southeast Asian countries and found to have 60% (w/w) sugar components. OPEFB was pretreated using the ammonia fibre expansion (AFEX) method and characterised physically by the Fourier transform infrared spectroscopy, X-ray diffraction and scanning electron microscopy. The results show that there were significant structural changes in OPEFB after the pretreatment step, and the sugar yield after enzymatic hydrolysis using a cocktail of Cellic Ctec2&reg; and Cellic Htec2&reg; increased from 0.15gg<SUP>-1</SUP> of OPEFB in the raw untreated OPEFB sample to 0.53gg<SUP>-1</SUP> of OPEFB in AFEX-pretreated OPEFB (i.e. almost a fourfold increase in sugar conversion), which enhances the economic value of OPEFB. A biohydrogen fermentability test of this hydrolysate was carried out using a locally isolated bacterium, Enterobacter sp. KBH6958. The biohydrogen yield after 72h of fermentation was 1.68mol H<SUB>2</SUB> per mol sugar. Butyrate, ethanol, and acetate were the major metabolites.

2016

Elsevier Applied Science

0960-8524

211

pp.200-208

10.1016/j.biortech.2016.02.135

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART75353278

Ammonia fibre expansion (AFEX); Oil palm empty fruit bunch (OPEFB) fibre; Biohydrogen; Oil palm residues; Biomass characterisation