초록
<P><B>Abstract</B></P> <P>The lignocellulosic agro-wastes are an attractive renewable resource in biorefinery for production of value-added platform chemicals and biofuels. The study describes use of different agro-wastes as substrate for production of lactic acid, a C3-platform chemical and high demand industrial product by <I>Lactobacillus brevis</I> in a one-pot bioprocess. The simultaneous saccharification and co-fermentation (SSCF) process was achieved by <I>L. brevis</I> governed fermentation of sugars, derived from saccharification of ionic liquid pretreated feedstocks by nanoimmobilized cellulase, which was further recovered and used for consecutive cycle. The lactic acid yields of 0.22, 0.49, 0.52 g/g were obtained from cottonseed cake, wheat straw and sugarcane bagasse, respectively. The ionic liquid-tolerant <I>L. brevis</I>, cellulolytic reusable nanoimmobilized enzyme coupled with valorization of renewable feedstocks points towards a holistic approach for future biorefineries with sustainable production of bioproducts.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Lignocellulosic agro-wastes exploited for lactic acid production. </LI> <LI> Simultaneous fermentation of hexose and pentose sugars by CCR negative <I>L. brevis</I>. </LI> <LI> Utilization of toxic deoiled cottonseed cake for cost-effective lactic acid production. </LI> <LI> One-pot bioprocess with IL pretreatment, immobilized cellulase and IL stable <I>L. brevis</I>. </LI> </UL> </P>