초록
Potential biohythane production from Sargassum sp. was evaluated in a two stage process. In the first stage, hydrogen dark fermentation was performed by Caldicellulosiruptor saccharolyticus. Sargassum sp. concentrations (VS) of 2.5, 4.9 and 7.4gL<SUP>-1</SUP> and initial inoculum concentrations (CDW) of 0.04 and 0.09gL<SUP>-1</SUP> of C. saccharolyticus were used in substrate/inoculum ratios ranging from 28 to 123. The end products from hydrogen production process were subsequently used for biogas production. The highest hydrogen and methane production yields, 91.3+/-3.3Lkg<SUP>-1</SUP> and 541+/-10Lkg<SUP>-1</SUP>, respectively, were achieved with 2.5gL<SUP>-1</SUP> of Sargassum sp. (VS) and 0.09gL<SUP>-1</SUP>of inoculum (CDW). The biogas produced contained 14-20% of hydrogen. Potential energy production from Sargassum sp. in two stage process was estimated in 242GJha<SUP>-1</SUP>yr<SUP>-1</SUP>. A maximum energy supply of 600EJyr<SUP>-1</SUP> could be obtained from the ocean potential area for macroalgae production.