BioChem - DOS

An organic solvent-tolerant alkaline lipase from Streptomyces sp. CS268 and its application in biodiesel production

Biotechnology and bioprocess engineering : Bbe

Mander, Poonam; Cho, Seung-Sik; Simkhada, Jaya Ram; Choi, Yun-Hee; Park, Da-Jeong; Ha, Jung-Wan; Yoo, Jin-Cheol

In an effort to identify a microbial lipase that can catalyze transesterification reactions used in biodiesel production, an organic solvent-tolerant lipase was purified from Streptomyces sp. CS268. The molecular weight of the purified lipase was estimated to be 37.5 kDa by SDS-PAGE. The lipase showed highest activity at a temperature of <TEX>$30^{\circ}C$</TEX> and pH 8.0 while it was stable in the pH range 4.0 ~ 9.0 and at temperatures <TEX>${\leq}50^{\circ}C$</TEX>. It showed the highest hydrolytic activity towards medium-length acyl chain p-nitrophenyl decanoate with <TEX>$K_m$</TEX> and <TEX>$V_{max}$</TEX> values of 0.59 mM and 319.5 mmol/mg/min, respectively. Also, the lipase showed non-position specificity for triolein hydrolysis. The purified lipase catalyzed transesterification reaction of soybean oil with methanol, suggesting that it can be a potential enzymatic catalyst for biodiesel production.

2012

Korean Society for Biotechnology and Bioengineering

1226-8372

1976-3816

17

1

pp.67-75

10.1007/s12257-011-0347-5

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=JAKO201224237131055

microbial lipase; organic solvent-tolerant; Streptomyces; biodiesel production