초록
<P><B>Abstract</B></P><P>Regiospecific oxyfunctionalization of renewable long chain fatty acids into industrially relevant C9 carboxylic acids has been investigated. One example was biocatalytic transformation of 10,12‐dihydroxyoctadecanoic acid, which was produced from ricinoleic acid ((9<I>Z</I>,12<I>R</I>)‐12‐hydroxyoctadec‐9‐enoic acid) by a fatty acid double bond hydratase, into (<I>R</I>)‐3‐hydroxynonanoic acid, 9‐hydroxynonanoic acid, and 1,9‐nonanedioic acid with a high conversion yield of ca. 70%. The biotransformation was driven by enzyme/whole‐cell biocatalysts, consisting of the esterase of <I>Pseudomonas fluorescens</I> and the recombinant <I>Escherichia coli</I> expressing the secondary alcohol dehydrogenase of <I>Micrococcus luteus</I>, the Baeyer‐Villiger monooxygenase of <I>Pseudomonas putida</I> KT2440 and the primary alcohol/aldehyde dehydrogenases of <I>Acinetobacter</I> sp. NCIMB9871. The high conversion yields and the high product formation rates over 20 U/g dry cells with insoluble reactants indicated that various (poly‐hydroxy) fatty acids could be converted into multi‐functional products via the simultaneous enzyme/whole‐cell biotransformations. This study will contribute to the enzyme‐based functionalization of hydrophobic substances.</P>