초록
<P>This work combines the thermostable alcohol dehydrogenase D (AdhD) from <I>Pyrococcus furiosus</I> and the organic electrocatalyst TEMPO to create a bifunctional catalyst that selectively oxidizes primary and secondary alcohols. The active sites function independently, can be switched on by changing reaction conditions, and can selectively oxidize a mixture of 1- and 2-butanol. The NAD<SUP>+</SUP>-dependent enzyme catalyses the secondary alcohol oxidation at a rate 3-fold faster than the primary alcohol, while the covalently attached 4-glycidyl-TEMPO oxidizes 1-butanol and has negligible activity toward 2-butanol. This hybrid catalytic approach has potential value for selective alcohol oxidations as well as other electrochemical and enzymatic multistep processes in energy conversion and chemical synthesis.</P><P>Graphic Abstract</P><P>TEMPO and AdhD were chemically conjugated into a bifunctional catalyst that selectively oxidizes primary and secondary alcohols.<BR/><IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c7cc08548f'/><BR/></P>