BioChem - DOS

Simplified process for ethanol production from sugarcane bagasse using hydrolysate-resistant Escherichia coli strain MM160

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Geddes, C.C.; Mullinnix, M.T.; Nieves, I.U.; Peterson, J.J.; Hoffman, R.W.; York, S.W.; Yomano, L.P.; Miller, E.N.; Shanmugam, K.T.; Ingram, L.O.

Hexose and pentose sugars from phosphoric acid pretreated sugarcane bagasse were co-fermented to ethanol in a single vessel (SScF), eliminating process steps for solid-liquid separation and sugar cleanup. An initial liquefaction step (L) with cellulase was included to improve mixing and saccharification (L+SScF), analogous to a corn ethanol process. Fermentation was enabled by the development of a hydrolysate-resistant mutant of Escherichia coli LY180, designated MM160. Strain MM160 was more resistant than the parent to inhibitors (furfural, 5-hydroxymethylfurfural, and acetate) formed during pretreatment. Bagasse slurries containing 10% and 14% dry weight (fiber plus solubles) were tested using pretreatment temperatures of 160-190<SUP>o</SUP>C (1% phosphoric acid, 10min). Enzymatic saccharification and inhibitor production both increased with pretreatment temperature. The highest titer (30g/L ethanol) and yield (0.21g ethanol/g bagasse dry weight) were obtained after incubation for 122h using 14% dry weight slurries of pretreated bagasse (180<SUP>o</SUP>C).

2011

Elsevier Applied Science

0960-8524

102

3

pp.2702-2711

10.1016/j.biortech.2010.10.143

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART55191959

Cellulase; Lignocellulose; SSF; L+SScF; Ethanol