초록
<P><B>Abstract</B><P><I>Piper longum</I> L. (long pepper) is an economically and industrially important medicinal plant. However, the characterization of its volatiles has only been analyzed by gas chromatography-mass spectrometry (GC-MS). In the present study, precise characterization of <I>P. longum</I> fruit volatiles has been performed for the first time through advanced two‐dimensional gas chromatography-time‐of‐flight spectrometry (GC×GC-TOFMS). A total of 146 constituents accounting for 93.79% were identified, of which 30 were reported for the first time. All these constituents were classified into alcohols (4.5%), alkanes (8.9%), alkenes (6.71%), esters (6.15%), ketones (0.58%), monoterpene hydrocarbons (1.64%), oxygenated monoterpenes (2.24%), sesquiterpene hydrocarbons (49.61%), oxygenated sesquiterpenes (13.03%), phenylpropanoid (0.23%), and diterpenes (0.2%). Among all the classes, sesquiterpene hydrocarbons were abundant, with germacrene‐D (2.87% ± 0.01%) as the major one, followed by 8‐heptadecene (2.69% ± 0.03%), <I>β</I>‐caryophyllene (2.43% ± 0.03%), <I>n</I>‐heptadecane (2.4% ± 0.04%), <I>n</I>‐pentadecane (2.11% ± 0.05%), and so forth. Further, 20 constituents were observed to be coeluted and separated precisely in the two‐dimensional column. The investigation provides an extensive metabolite profiling of <I>P. longum</I> fruit volatiles, which could be helpful to improve its therapeutic potential.</P></P>