초록
<P><B>Abstract</B></P> <P>The aim of this work is to show the feasibility of a one-pot reaction in three phase hybrid catalysis, one step being an oxidation. The model reaction proposed is a two-step reaction: an enzymatic catalysed isomerisation of fructose to glucose followed by a heterogeneously catalysed oxidation of glucose to gluconic acid. The reaction medium required for the enzymatic step was shown to be compatible with the oxidation catalyst. Kinetic parameters were estimated from experimental data to run simulation using an isothermal one-pot slurry model. Finally, a one-pot test was performed which showed that combining both steps is possible.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A new methodology is proposed to characterize reactor for hybrid catalysis. </LI> <LI> Combination of enzymatic fructose isomerization with glucose oxidation studied as model system. </LI> <LI> Kinetic parameters are obtained without and with combination. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>