초록
<P><B>Abstract</B></P> <P>To enhance the stability of lipases, the additive method is attractive because of its high efficiency and simplicity, of which β-cyclodextrin (β-CD), as an additive has attracted attention. In the present study, the effects of β-CD on enzymatic hydrolysis of hemp seed oil by lipases were investigated. The stability, spectroscopy, and reusability of C<I>andida</I> sp.99–125 were compared by the addition of β-CD, respectively. The results showed that <I>Candida</I> sp.99–125 could produce the highest yield of α-linolenic acid (ALA, 18.27%, w/w) in the presence of β-CD after 24h, and the thermal stability of lipase was also improved. The UV spectroscopy showed that the absorbance of lipase decreased with increasing concentrations of β-CD and the fluorescence result was similar. Moreover, the reusability of lipase with β-CD was better than free lipase. This study revealed that β-CD could increase hydrolysis activity and stability of the lipase, thereby improving the hydrolytic process of hemp seed oil by <I>Candida</I> sp.99–125.</P> <P><B>Highlights</B></P> <P> <UL> <LI> β-cyclodextrin as a additive was used in hydrolysis reaction. </LI> <LI> β-cyclodextrin could improve the thermal stability of <I>Candida</I> sp.99–125. </LI> <LI> The spectroscopy showed that β-CD could form inclusion complexes with lipase. </LI> <LI> β-cyclodextrin could improve the hydrolysis activity of <I>Candida</I> sp.99–125. </LI> </UL> </P>