BioChem - DOS

Determination of organic acid impurities in lactic acid obtained by fermentation of sugarcane juice

Journal of chromatography A

Qureshi, Mohd.S.; Bhongale, S.S.; Thorave, A.K.

Lactic acid produced by fermentation process mostly contains a number of aliphatic carboxylic acids as impurities. In this work, carboxylic acid impurities in lactic acid samples from a number of sources were determined at ppm levels. A simple HPLC method was developed that utilized a new generation polar embedded reverse phase, 20mM phosphate buffer at pH 2.20 (+/-0.05) and UV detection at 210nm. The method enabled quantitative analysis of the above acids in lactic acid matrix. The experimental conditions for column temperature, mobile phase pH and flow rate were optimized. A detailed validation of the method was performed for linearity, precision, accuracy, selectivity, limit of detection (LOD), limit of quantitation (LOQ), ruggedness and repeatability and reproducibility (R&R).

2011

Elsevier

0021-9673

1873-3778

1218

40

pp.7147-7157

10.1016/j.chroma.2011.08.025

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART57343282

Lactic acid; Organic acid impurities; Sugarcane juice fermentation; Polar embedded RP-HPLC; Method validation