<P><B>Abstract</B></P> <P>Enzymatic hydrolysis of <I>Saccharina latissima</I> with laminarinase was compared to hydrolysis with different combinations of cellulase and hemicellulase enzyme mixtures. The hemicellulase mixture resulted in similar release of glucose, while the cellulase mixture released 40% more glucose than laminarinase alone. The combination of a laminarinase augmented with a cellulase mixture resulted in a 53% increase of glucose release from <I>S. latissima</I> than laminarinase. Increasing biomass loading above 4% (w/v) reduced the sugar yield. Resulting macroalgae hydrolysates were used as a carbon source for the production of pinene, making use of a novel two plasmid <I>Escherichia coli</I> system. The macroalgal hydrolysates were suitable for the novel microbial production of pinene with no further treatment and/or purification.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>S. latissima</I> was used as a sugar source for fermentation of pinene. </LI> <LI> A cellulase and laminarinase mixture releases more glucose than laminarinase. </LI> <LI> Hydrolysates were used to produce pinene with a novel <I>Escherichia coli</I> system. </LI> </UL> </P>