BioChem - DOS

Lipase-catalyzed transesterification of ethyl formate to octyl formate

Journal of molecular catalysis. B, Enzymatic

Janssen, L.M.G.; van Oosten, R.; Paul, C.E.; Arends, I.W.C.E.; Hollmann, F.

The preparation of octyl formate via lipase-catalyzed transesterification of ethyl formate with 1-octanol is demonstrated. To shift the equilibrium of the reaction, ethyl formate was added in surplus but could be partially recovered for subsequent reactions. The same was true for the biocatalyst (Novo435), which could be reused at least 27 times. This method gives simple access to a hydrophobic formic acid ester, which can be used as a reactive organic phase in biocatalytic redox reactions. The enzymatically prepared octyl formate can be utilized by formate dehydrogenase to regenerate NADH from NAD<SUP>+</SUP>.

2014

Elsevier

1381-1177

1873-3158

105

pp.7-10

10.1016/j.molcatb.2014.03.016

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART69129492

Transesterification; NADH regeneration; Formate dehydrogenase; Lipase