초록
<P><B>Abstract</B></P><P>Direct and regiospecific amino‐functionalization of non‐activated carbon could be achieved using one recombinant microbial catalyst. The presented proof of concept shows that heterologous pathway engineering allowed the construction of a whole‐cell biocatalyst catalyzing the terminal amino‐functionalization of fatty acid methyl esters (e.g., dodecanoic acid methyl ester) and alkanes (e.g., octane). By coupling oxygenase and transaminase catalysis <I>in vivo</I>, both substrates are converted with absolute regiospecificity to the terminal amine <I>via</I> two sequential oxidation reactions followed by an amination step. Such demanding chemical three‐step reactions achieved with a single catalyst demonstrate the tremendous potential of whole‐cell biocatalysts for the production of industrially relevant building blocks.</P>