초록
<P><B>Abstract</B></P><P>The optimum conditions for the production of <SMALL>L</SMALL>-arabinose from debranched arabinan were determined to be pH 6.5, 75°C, 20gl<SUP>−1</SUP> debranched arabinan, 42Uml<SUP>−1</SUP> endo-1,5-α-<SMALL>L</SMALL>-arabinanase, and 14Uml<SUP>−1</SUP> α-<SMALL>L</SMALL>-arabinofuranosidase from <I>Caldicellulosiruptor saccharolyticus</I> and the conditions for sugar beet arabinan were pH 6.0, 75°C, 20gl<SUP>−1</SUP> sugar beet arabinan, 3Uml<SUP>−1</SUP> endo-1,5-α-<SMALL>L</SMALL>-arabinanase, and 24Uml<SUP>−1</SUP> α-<SMALL>L</SMALL>-arabinofuranosidase. Under the optimum conditions, 16gl<SUP>−1</SUP> <SMALL>L</SMALL>-arabinose was obtained from 20gl<SUP>−1</SUP> debranched arabinan or sugar beet arabinan after 120min, with a hydrolysis yield of 80% and a productivity of 8gl<SUP>−1</SUP>h<SUP>−1</SUP>. This is the first reported trial for the production of <SMALL>L</SMALL>-arabinose from the hemicellulose arabinan by the combined use of endo- and exo-arabinanases.</P>