초록
<P><B>Abstract</B></P> <P>Although L-Serine has wide applications and great market potential, its application is limited by its high cost and low yields. Microbial factories have many advantages for producing L-serine from renewable carbon resources. To achieve L-serine production with high efficiency, three enzymes (SerB, SerC, and EamA) were physically re-localized by using a scaffold system GBD:SH3:PDZ. Such strategy was highly effective in improving the production of L-serine in <I>Escherichia coli</I>. The highest concentration of L-serine harvested was at 3.8 g/l after 48 h in strain <I>E. coli</I> with the scaffold GBD:SH3:PDZ at ratio of 1:2:1, reaching a high yield of 0.24 mol/mol glucose.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Protein scaffold PSAT, PSP, and EamA for the production of L-serine. </LI> <LI> Overexpression with synthesis scaffold protein harvested 2.3 g/l L-serine. </LI> <LI> Highest L-serine titer reached 3.18 g/l, which is 23.7% mass yield. </LI> </UL> </P>