Effect of membrane-bound aldehyde dehydrogenase-encoding gene disruption on glyceric acid production in Gluconobacter oxydans

BioChem - DOS

Search

메타 데이터

바이오화학분류

- 바이오정밀화학
  1. 용매
- 화장품용 기능성소재
  1. 계면활성제⁄증점제
- 의료용 화학소재
  1. 식품첨가제

논문

Effect of membrane-bound aldehyde dehydrogenase-encoding gene disruption on glyceric acid production in Gluconobacter oxydans

학술지

Journal of oleo science

저자명

Habe, Hiroshi; Sato, Shun; Fukuoka, Tokuma; Kitamoto, Dai; Sakaki, Keiji

초록

<P>Gluconobacter oxydans IFO12528 is able to produce glyceric acid (GA) from glycerol through the action of a membrane-bound alcohol dehydrogenase (mADH), which is required for GA production. To determine whether membrane-bound aldehyde dehydrogenase (mALDH) also plays a role in GA production in G. oxydans, we constructed an aldH-disrupted mutant of G. oxydans (??aldH). ??aldH was unable to produce acetic acid from ethanol, but was able to produce GA at a level approximately half that of the wildtype strain, suggesting the involvement of another ALDH in GA production. We also investigated the enantiomeric composition of GA produced by the IFO12528 and ??aldH strains. No difference in GA composition was evident in the ??aldH mutant, with ~73% D-GA enantiomeric excess observed in both strains.</P>

발행연도

2014

발행기관

Japan Oil Chemists' Society

ISSN

1345-8957

권

호

페이지

pp.953-957

DOI

10.5650/jos.ess14112