BioChem - DOS

Cascade hydrolysis and fermentation of corn stover for production of high titer gluconic and xylonic acids

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Hou, Weiliang; Zhang, Maofen; Bao, Jie

<P><B>Abstract</B></P> <P>Simultaneous saccharification and fermentation (SSF) is an efficient fermentation operation in lignocellulose biorefining. However, SSF may not be applicable when the pH values of hydrolysis and fermentation do not match, or the strong intermediate inhibitors on cellulase activity are generated. This study proposed a cascade hydrolysis and fermentation (CHF) process for cellulosic gluconic acid fermentation to overcome the inhibition of the intermediate glucono-&gamma;-lactone on cellulase activity. The pretreated and detoxified corn stover feedstock was enzymatically hydrolyzed into hydrolysate slurry, then gluconic acid and xylonic acid fermentations were directly conducted by inoculating <I>Gluconobacter oxydans</I> strain without solid/liquid separation. The sugar loss and energy consumption were effectively avoided by moving the solid/liquid separation into the fermentation stage. The experiments and the techno-economic analysis show that the CHF is simple and cost effective fermentation operation when SSF is not applicable.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Cascade hydrolysis and fermentation (CHF) without S/L separation was proposed. </LI> <LI> CHF is applied to lignocellulose biorefinery fermentations when SSF is not applicable. </LI> <LI> A record high cellulosic product of gluconic and xylonic acids was obtained. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

2018

Elsevier

0960-8524

264

pp.395-399

10.1016/j.biortech.2018.06.025

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART85395694

Cascade hydrolysis and fermentation (CHF); Lignocellulose; Gluconic acid; Glucono-γ-lactone; Solid/liquid separation