초록
<P><B>Abstract</B></P> <P>In this study, a novel inoculation method to mitigate the inhibition of 5-hydroxymethylfurfural (5-HMF) is proposed. Acid algae hydrolysate containing 1.5 g 5-HMF/L and 15 g hexose/L hexose was fed to a continuous fixed bed reactor (C-FBR) partially packed with hybrid-immobilized beads. The inoculation method enabled a high rate of H<SUB>2</SUB> production, due to the reduction of 5-HMF inhibition and enhanced biofilm formation. Maximum hydrogen production was achieved at a hydraulic retention time of 6 h with a hydrogen production rate (HPR) of 20.0 ± 3.3 L H<SUB>2</SUB>/L-d and a hydrogen yield (HY) of 2.3 ± 0.4 mol H<SUB>2</SUB>/mol hexose <SUB>added</SUB>. Butyrate and acetate were the major soluble metabolic products released during fermentation. Quantitative real-time polymerase chain reaction analysis revealed that <I>Clostridium butyricum</I> comprised 94.3% of the total bacteria, which was attributed to the high rate of biohydrogen production.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Pore of support material was packed with hybrid immobilized beads. </LI> <LI> The new inoculation method mitigated the inhibition of 5-HMF on bio-H<SUB>2</SUB>. </LI> <LI> H<SUB>2</SUB> production of 20.0 L/L-d was achieved with 1.5 g 5-HMF/L. </LI> </UL> </P>