BioChem - DOS

Economically enhanced succinic acid fermentation from cassava bagasse hydrolysate using Corynebacterium glutamicum immobilized in porous polyurethane filler

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Shi, X.; Chen, Y.; Ren, H.; Liu, D.; Zhao, T.; Zhao, N.; Ying, H.

An immobilized fermentation system, using cassava bagasse hydrolysate (CBH) and mixed alkalis, was developed to achieve economical succinic acid production by Corynebacterium glutamicum. The C. glutamicum strains were immobilized in porous polyurethane filler (PPF). CBH was used efficiently as a carbon source instead of more expensive glucose. Moreover, as a novel method for regulating pH, the easily decomposing NaHCO<SUB>3</SUB> was replaced by mixed alkalis (NaOH and Mg(OH)<SUB>2</SUB>) for succinic acid production by C. glutamicum. Using CBH and mixed alkalis in the immobilized batch fermentation system, succinic acid productivity of 0.42gL<SUP>-1</SUP>h<SUP>-1</SUP> was obtained from 35gL<SUP>-1</SUP> glucose of CBH, which is similar to that obtained with conventional free-cell fermentation with glucose and NaHCO<SUB>3</SUB>. In repeated batch fermentation, an average of 22.5gL<SUP>-1</SUP> succinic acid could be obtained from each batch, which demonstrated the enhanced stability of the immobilized C. glutamicum cells.

2014

Elsevier Applied Science

0960-8524

174

pp.190-197

10.1016/j.biortech.2014.09.137

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART70650940

Cassava bagasse hydrolysate; Corynebacterium glutamicum; Mixed alkaline neutralizers; Porous polyurethane filler (PPF); Succinic acid