BioChem - DOS

Solid state fermentation pilot-scaled plug flow bioreactor, using partial least square regression to predict the residence time in a semicontinuous process

Biochemical engineering journal

Carboué , Quentin; Ré bufa, Catherine; Dupuy, Nathalie; Roussos, Sevastianos; Bombarda, Isabelle

<P><B>Abstract</B></P> <P>Plug flow bioreactor (PFB) used in solid state fermentation gives the possibility to have semicontinuous culture. However, it is complicated to follow a single particle during its residence inside the bioreactor and to therefore study precisely the culture process all along the device. In this study, semicontinuous production of fungal compounds was successfully obtained by cultivating <I>Aspergillus niger</I> with a PFB prototype. Kinetic productions of the same metabolites along the bioreactor were obtained at laboratory scale to predict the residence time of the particles inside the PFB from Partial Least Square (PLS). This original methodology allowed (1) the comparison between the production kinetics at the two production scales (pilot and laboratory) and (2) the observation of eventual changes following the scale-up of the process offering a good overall insight of the PFB performance.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Semicontinuous production of fungal compounds was obtained with a Plug Flow Bioreactor (PFB) prototype. </LI> <LI> Productions of same metabolites obtained over time at lab scale were used to build a Partial Least Square (PLS) model. </LI> <LI> A residence time of solid particles was associated to each PFB compartment using the PLS model. </LI> <LI> The PFB prototype was suitable to continuously produce fungal secondary metabolites. </LI> <LI> PLS was an innovative approach to evaluate the scale-up of the metabolite production. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

2019

Elsevier

publisher-specific-oa

1369-703x

149

pp.107248

10.1016/j.bej.2019.107248

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART97555283

http://manuscript.elsevier.com/S1369703X19301755/pdf/S1369703X19301755.pdf

Plug flow bioreactor; Solid state fermentation; Partial least square regression; Residence time; Aspergillus niger