BioChem - DOS

Biohydrogen production from palm oil mill effluent (POME) by two stage anaerobic sequencing batch reactor (ASBR) system for better utilization of carbon sources in POME

International journal of hydrogen energy

Maaroff, Rizal Muzhafar; Md Jahim, Jamaliah; Azahar, Azratul Madihah; Abdul, Peer Mohamed; Masdar, Mohd Shahbudin; Nordin, Darman; Abd Nasir, Muhammad Azri

<P><B>Abstract</B></P> <P>The production of biohydrogen through dark fermentation of palm oil mill effluent (POME) was evaluated in two-stages of biohydrogen in an anaerobic sequencing batch reactor (ASBR) system using enriched mixed culture for the first time. This study attempts to examine the effect of HRT and its interaction behavior with the solid retention time (SRT), and the sugar consumption. The effluent after discharged from the thermophilic reactor contained 7.61 g/L TC and 22.87 g/L TSS was fed to the secondary mesophilic reactor system. Results indicated that the overall sugar consumption reached 88.62% at the optimum HRT of 12 h with the SRT set to 20 h. The optimum hydrogen yield and HPR in the thermophilic stage were 2.99 mol H<SUB>2</SUB>/mol-sugar and 8.54 mmol H<SUB>2</SUB>/L&middot;h respectively, while for the mesophilic stage were 1.19 mol H<SUB>2</SUB>/mol-sugar and 1.47 mmolH<SUB>2</SUB>/L&middot;h respectively. The overall HPR showed an improvement and increase from 8.54 mmol H<SUB>2</SUB>/L&middot;h to 10.34 mmol H<SUB>2</SUB>/L.h. Microbial community analysis of mixed culture in the two-stage thermophilic (55.0 &deg;C) and mesophilic (37.0 &deg;C) ASBR reactor was dominated by <I>Thermoanaerobacterium</I> sp. based on the PCR-DGGE technique.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Reutilization of residual effluent from first reactor enhanced biohydrogen recovery. </LI> <LI> The cellular biomass retention time were varied with different HRTs. </LI> <LI> The PCR-DGGE technique were used to interpret particular microbial community. </LI> <LI> Conversion of the unutilized fermentable sugar to VFA for up to 90.0%. </LI> </UL> </P>

2019

Elsevier

0360-3199

1879-3487

44

6

pp.3395-3406

10.1016/j.ijhydene.2018.06.013

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART94847914

Thermophilic-mesophilic system; Two-stage; Renewable energy; Mixed culture; POME; Microbial community analysis