BioChem - DOS

Effective feather degradation and keratinase production by Bacillus pumilus GRK for its application as bio-detergent additive

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Ramakrishna Reddy, M.; Sathi Reddy, K.; Ranjita Chouhan, Y.; Bee, Hameeda; Reddy, Gopal

<P><B>Abstract</B></P> <P>An effecient feather-degrading bacterium was isolated from poultry dumping yard and identified as <I>Bacillus pumilus</I> GRK based on 16S rRNA sequencing. Complete feather degradation (98.3&plusmn;1.52%) with high keratinase production (373&plusmn;4 U/ml) was observed in 24h under optimized conditions (substrate 1% (w/w); inoculum size 4% (v/v); pH 10; 200rpm at 37&deg;C) with feathers as sole carbon and nitrogen source in tap water. The fermented broth was enriched with amino acids like tryptophan (221.44&micro;g/ml), isoleucine (15.0&micro;g/ml), lysine (10.81&micro;g/ml) and methionine (7.24&micro;g/ml) suggesting its potential use as feed supplement. The keratinase produced was a detergent stable serine protease and its activity was further enhanced by Ca<SUP>+2</SUP> and Mg<SUP>+2</SUP>. <I>Bacillus pumilus</I> GRK keratinase was successfully utilised as bioadditive in detergent formulations for removing the blood stains from cloth without affecting its fiber and texture.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Faster feather degradation and high keratinase production by <I>B. pumilus</I> GRK. </LI> <LI> Poultry feather used as sole carbon and nitrogen source for keratinase production. </LI> <LI> Important amino acids in fermented broth suggest its use as animal feed supplement. </LI> <LI> Crude enzyme has great potential as bio-detergent additive. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>

2017

Elsevier

0960-8524

243

pp.254-263

10.1016/j.biortech.2017.06.067

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART78577586

Feather degradation; Keratinase; Bacillus pumilus; Bio-detergent