BioChem - DOS

Efficient hydrolysis of corncob residue through cellulolytic enzymes from Trichoderma strain G26 and L-lactic acid preparation with the hydrolysate

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Xie, L.; Zhao, J.; Wu, J.; Gao, M.; Zhao, Z.; Lei, X.; Zhao, Y.; Yang, W.; Gao, X.; Ma, C.; Liu, H.; Wu, F.; Wang, X.; Zhang, F.; Guo, P.; Dai, G.

To prepare fermentable hydrolysate from corncob residue (CCR), Trichoderma strain G26 was cultured on medium containing CCR for production of cellulolytic enzymes through solid-state fermentation (SSF), resulting in 71.3IU/g (FPA), 136.2IU/g (CMCase), 85.1IU/g (β-glucosidase) and 11,344IU/g (xylanase), respectively. Through a three-stage saccharification strategy, CCR was hydrolyzed by the enzymatic solution (6.5FPU/ml) into fermentable hydrolysate containing 60.1g/l glucose (81.2% cellulose was converted at solid loading of 12.5%), 21.4% higher than that by the one-stage method. And then the hydrolysate was used to produce l-lactic acid by a previous screened strain Bacillus coagulans ZX25 in the submerged fermentation. 52.0g/l l-lactic acid was obtained after fermentation for 44h, with 86.5% glucose being converted to l-lactic acid. The results indicate that the strains and the hydrolysis strategy are promising for commercial production of l-lactic acid from CCR and other biomass.

2015

Elsevier Applied Science

0960-8524

193

pp.331-336

10.1016/j.biortech.2015.06.101

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART72402069

Corncob residue; Solid-state fermentation; Saccharification; Trichoderma longibrachiatum; Cellulase