BioChem - DOS

Influence of airflow intensity on phytase production by solid-state fermentation

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Rodriguez-Fernandez, D.E.; Rodriguez-Leon, J.A.; de Carvalho, J.C.; Karp, S.G.; Sturm, W.; Parada, J.L.; Soccol, C.R.

Phytase production by Aspergillus niger F3 by solid state fermentation (SSF) on citrus peel was evaluated at pilot scale under different aeration conditions. The best airflow intensity was 1VkgM (Lair kgmedium<SUP>-1</SUP>min<SUP>-1</SUP>), which allowed to produce 65units of phytases per gram in dry basis (65Ug<SUP>-1</SUP>d.b.) as it removed the metabolic heat generated by the microorganism, Agitation did not improve heat removal. Airflow intensity was considered as scale-up criterion. When the airflow intensity was maintained at 1VkgM for SSF with 2 and 20kg of medium, the kinetics parameters for biomass and enzyme concentration at the end of fermentation differed by less than 2. The air flow intensity was required to maintain the temperature and cool the SSF and to provide oxygen for microbial growth. Air flow intensity is a key a factor that must be considered when scale-up of SSF is attempted.

2012

Elsevier Applied Science

publisher-specific-oa

0960-8524

118

pp.603-606

10.1016/j.biortech.2012.05.032

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART63386341

Scale-up; Metabolic heat; Airflow intensity; Solid-state fermentation; Phytases