초록
<P><B>Highlights</B></P><P>► The growth of <I>Rhodotorula glutinis</I> has been shown to be enhanced by irradiation. ► Different LEDs’ wavelengths do not have a significant impact on the biomass. ► Inhibitive metabolic products accumulated in the broth, which might impede growth. ► The combing of microfiltration and the irradiation could effectively enhance the growth of cells.</P> <P><B>Abstract</B></P><P>The results of this study indicate that the irradiation could enhance the cells growing of <I>Rhodotorula glutinis</I> to 54.2±1.6g/L as compared to the control (without irradiation) of 38.3±1.2g/L. However, different wavelength of LEDs’ (red, green, blue and white) had no significant impacts on the growth and on the lipid content. The accumulation of potential inhibitive metabolic products probably impedes growth, which restricts more biomass accumulated in the fed-batch operation with irradiation. The combining of the fed-batch operation with irradiation and microfiltration can successfully improve the growth of <I>R. glutinis</I> to the maximum of 72.4±0.6g/L and 51.2±4.9% of lipid content obtained. Conclusively, the integration process of a fed-batch operation, irradiation and microfiltration can effectively enhance cell growth in <I>R. glutinis</I>, without any reimbursement of lipid contents. This finding might be useful when applied to the commercialized cultivation of <I>R. glutinis</I> for biodiesel production.</P>