Uncharacterized Escherichia coli proteins YdjA and YhjY are related to biohydrogen production

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논문

Uncharacterized Escherichia coli proteins YdjA and YhjY are related to biohydrogen production

학술지

International journal of hydrogen energy

저자명

Mohd Yusoff, M.Z.; Maeda, T.; Sanchez-Torres, V.; Ogawa, H.I.; Shirai, Y.; Hassan, M.A.; Wood, T.K.

초록

Biohydrogen has gained importance as an alternative energy source, and advances in molecular biology and biotechnology have raised the quality and efficiency of biohydrogen production from various microorganisms and substrates. Here, Escherichia coli proteins YdjA and YhjY have been identified as essential in biohydrogen production from glucose. The mutations ydjA and yhjY reduced biohydrogen productivity compared to the parent strain from 40 to 4 and 29 μmol/mg protein, respectively. Through transcription analysis, it was determined that YdjA and YhjY are positive effectors of the FHL complex since their inactivation repressed fhlA. In addition, the FHL expression of the repressor gene, hycA, increased for the ydjA mutant, so YdjA reduces transcription of the HycA repressor. Hence, two new proteins have been identified that are important for biohydrogen production.

발행연도

2012

발행기관

Pergamon Press ; Elsevier Science Ltd

ISSN

0360-3199

ISSN

1879-3487

권

호

페이지

pp.17778-17787