BioChem - DOS

Novel strategy to improve vanillin tolerance and ethanol fermentation performances of Saccharomycere cerevisiae strains

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Zheng, Dao-Qiong; Jin, Xin-Na; Zhang, Ke; Fang, Ya-Hong; Wu, Xue-Chang

<P><B>Abstract</B></P> <P>The aim of this work was to develop a novel strategy for improving the vanillin tolerance and ethanol fermentation performances of <I>Saccharomyces cerevisiae</I> strains. Isogeneic diploid, triploid, and tetraploid <I>S. cerevisiae</I> strains were generated by genome duplication of haploid strain CEN.PK2-1C. Ploidy increments improved vanillin tolerance and diminished proliferation capability. Antimitotic drug methyl benzimidazol-2-ylcarbamate (MBC) was used to introduce chromosomal aberrations into the tetraploid <I>S. cerevisiae</I> strain. Interestingly, aneuploid mutants with DNA contents between triploid and tetraploid were more resistant to vanillin and showed faster ethanol fermentation rates than all euploid strains. The physiological characteristics of these mutants suggest that higher bioconversion capacities of vanillin and ergosterol contents might contribute to improved vanillin tolerance. This study demonstrates that genome duplication and MBC treatment is a powerful strategy to improve the vanillin tolerance of yeast strains.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A novel strategy to improve vanillin tolerance was proposed. </LI> <LI> Triploid yeast strain is more tolerant to vanillin than other euploid strains. </LI> <LI> MBC treatment of polyploidy strains generated aneuploidy mutants. </LI> <LI> Certain aneuploid mutants showed faster fermentation rates than euploid strains. </LI> <LI> Bioconversion ability and ergosterol content contribute to vanillin tolerance. </LI> </UL> </P>

2017

Elsevier

0960-8524

231

pp.53-58

10.1016/j.biortech.2017.01.040

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART77419847

Bioethanol; Vanillin; Saccharomyces cerevisiae; Tolerance; Ploidy