Bioethanol production from the yeast Yarrowia lipolytica biomass was studied. The effects of temperature (90-150<SUP>o</SUP>C) and H<SUB>2</SUB>SO<SUB>4</SUB> concentration (2-15%w/w) on the saccharification of biomass at a hydrolysis time of 1h were investigated. A maximum glucose concentration of 35.89g/L can be produced from defatted biomass using 6% H<SUB>2</SUB>SO<SUB>4</SUB> at 120<SUP>o</SUP>C. Subcritical water (SCW) pretreatment has negligible effect on maximizing glucose yield. Only 14.53g/L glucose can be produced using 6% H<SUB>2</SUB>SO<SUB>4</SUB> at 120<SUP>o</SUP>C if un-defatted biomass was used. The highest ethanol concentration achieved was 13.39g/L with a corresponding ethanol yield of 0.084g/g dry biomass (0.38g ethanol/g glucose).