초록
This paper reports on processing options for the conversion of feedlot cattle manures into composite sugars for ethanol fermentation. Small-scale anaerobic digestion trials revealed that the process significantly reduces the content of glucan and xylan (ca. 70%) without effecting lignin. Moreover, anaerobic digestate (AD) fibres were poor substrates for cellulase (Cellic® CTec 2) saccharification, generating a maximum combined sugar yield of ca. 12% per original dry weight. Dilute acid pretreatment and enzyme saccharification of raw manures significantly improved total sugar recoveries, totalling 264mg/g (79% theoretical). This was attained when manures were pretreated with 2.5% H<SUB>2</SUB>SO<SUB>4</SUB> for 90min at 121<SUP>o</SUP>C and saccharified with 50FPU CTec 2/g glucan. Saccharomycescerevisiae efficiently fermented crude hydrolysates within 6h, yielding 7.3g/L ethanol, representing glucose to ethanol conversion rate of 70%. With further developments (i.e., fermentation of xylose), this process could deliver greater yields, reinforcing its potential as a biofuel feedstock.