BioChem - DOS

Engineered biosynthesis of 5/5/6 type polycyclic tetramate macrolactams in an ikarugamycin (5/6/5 type)-producing chassis

Organic letters

Jin, Hongbo; Zhang, Wenjun; Zhang, Guangtao; Zhang, Liping; Liu, Wei; Zhang, Changsheng

<P>Combinatorial biosynthesis of 5/5/6 type polycyclic tetramate macrolactams (PoTeMs) was achieved in an engineered ikarugamycin (5/6/5 type) producer by introducing a set of 5/5/6 type PoTeM biosynthetic genes from <I>Streptomyces griseus</I>. This study supports the idea that PoTeMs share a common polyene tetramate precursor generated by the hybrid polyketide synthase/nonribosomal peptide synthetase enzymes. The X-ray crystal structure of pactamide G (<B>7</B>) sets an example for resolving the absolute configuration of 5/5/6 type PoTeMs.</P><BR>[FIG OMISSION]</BR>

2020

American Chemical Society

1523-7060

22

5

pp.1731-1735

10.1021/acs.orglett.9b04672

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART98899904