Prakash, G.; Varma, A.J.; Prabhune, A.; Shouche, Y.; Rao, M.
초록
A thermotolerant yeast capable of fermenting xylose to xylitol at 40<SUP>o</SUP>C was isolated and identified as a strain of Debaryomyces hansenii by ITS sequencing. This paper reports the production of xylitol from d-xylose and sugarcane bagasse hemicellulose by free and Ca-alginate immobilized cells of D. hansenii. The efficiency of free and immobilized cells were compared for xylitol production from d-xylose and hemicellulose in batch culture at 40<SUP>o</SUP>C. The maximum xylitol produced by free cells was 68.6g/L from 100g/L of xylose, with a yield of 0.76g/g and volumetric productivity 0.44g/L/h. The yield of xylitol and volumetric productivity were 0.69g/g and 0.28g/L/h respectively from hemicellulosic hydrolysate of sugarcane bagasse after detoxification with activated charcoal and ion exchange resins. The Ca-alginate immobilized D. hansenii cells produced 73.8g of xylitol from 100g/L of xylose with a yield of 0.82g/g and volumetric productivity of 0.46g/L/h and were reused for five batches with steady bioconversion rates and yields.