초록
<P><B>Abstract</B></P><P>To convert waste disposable wooden chopsticks, cellulosic waste biomass, into ethanol efficiently, the effectiveness of pretreatments, i.e. ball-milling and steam explosion, for the enzymatic hydrolysis of aspen chopsticks and the dependence of substrate concentration using pretreated aspen chopsticks for the ethanol production were studied. In the enzymatic saccharification of ball-milled chopsticks, the maximum amount of glucose, 520mg-glucoce/g-dry aspen chopsticks, was obtained from the chopsticks ball-milled for 60min. On the other hand, the maximum amount of glucose, 598mg-glucoce/g-dry aspen chopsticks, was obtained using steam-exploded aspen chopsticks treated at a steam pressure of 25atm and a steaming time of 5min. Simultaneous saccharification and fermentation (SSF) of the steam-exploded chopsticks by Meicelase and <I>Saccahromyces cerevisiae</I> AM12 provided 241mg-ethanol/g-dry aspen chopsticks, corresponding to 79% of theoretical ethanol yield.</P>