BioChem - DOS

Fed-batch fermentation for enhanced lactic acid production from glucose/xylose mixture without carbon catabolite repression

Journal of bioscience and bioengineering

Abdel-Rahman, M.A.; Xiao, Y.; Tashiro, Y.; Wang, Y.; Zendo, T.; Sakai, K.; Sonomoto, K.

There has been tremendous growth in the production of optically pure l-lactic acid from lignocellulose-derived sugars. In this study, Enterococcus mundtii QU 25 was used to ferment a glucose/xylose mixture to l-lactic acid. Maintenance of the xylose concentration at greater than 10 g/L achieved homo-lactic acid fermentation and reduced the formation of byproducts. Furthermore, carbon catabolite repression (CCR) was avoided by maintaining the glucose concentration below 25 g/L; therefore, initial concentrations of 25 g/L glucose and 50 g/L xylose were selected. Supplementation with 5 g/L yeast extract enhanced the maximum xylose consumption rate and consequently increased lactic acid production and productivity. Finally, a 129 g/L lactic acid without byproducts was obtained with a maximum lactic acid productivity of 5.60 g/(L.h) in fed-batch fermentation with feeding a glucose/xylose mixture using ammonium hydroxide as the neutralizing agent. These results indicate a potential for lactic acid production from glucose and xylose as the main components of lignocellulosic biomasses.

2015

Society for Bioscience and Bioengineering, Japan ; Distributed outside Japan by Elsevier Science

1389-1723

1347-4421

119

2

pp.153-158

10.1016/j.jbiosc.2014.07.007

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART71246575

Glucose/xylose mixture; l-Lactic acid production; Fed-batch fermentation; Carbon catabolite repression; Enterococcus mundtii QU 25