BioChem - DOS

Optimisation of lipase production by a mutant of Candida antarctica DSM-3855 using response surface methodology

International journal of food science & technology

Liu, Junhong; Zhang, Yuanyuan

<P><B>Summary</B></P><P>Response surface methodology (RSM) was used to determine the optimum cultivation conditions for lipase production by a mutant strain of <I>Candida antarctica</I> DSM&#8208;3855. Among four variables, initial pH, soybean meal, and temperature were identified as significant variables for lipase production by full factorial design. A mathematical model was developed to investigate the influences of three factors on lipase activity and to predict their optimum value. According to RSM, the optimum cultivation conditions for the maximum lipase production were found to be: initial pH 6.0, soybean meal 3.38% (w/v), and temperature 26&emsp;&deg;C. Under the optimum conditions, the maximum lipase yields of 27.34 U&emsp;mL<SUP>&minus;1</SUP> was obtained after 58&emsp;h of cultivation in a 15&emsp;L fermenter.</P>

2011

Blackwell Publishing Ltd

0950-5423

1365-2621

46

4

pp.695-701

10.1111/j.1365-2621.2011.02548.x

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART56784932

Candida antarctica; cultivation conditions; lipase; optimisation; response surface methodology