BioChem - DOS

Enzymatic hydrolysis of olive wastewater for hydroxytyrosol enrichment

Bioresource technology : biomass, bioenergy, biowastes, conversion technologies, biotransformations, production technologies

Khoufi, Sonia; Hamza, Manel; Sayadi, Sami

<P><B>Highlights</B></P><P>&#x025BA; <I>Aspergillus niger</I> enzyme preparation is used for olive wastewater (OW) hydrolysis. &#x025BA; Large amount of free hydroxytyrosol (HT) is released. &#x025BA; &beta;-Glucosidase is the key enzyme for HT production. &#x025BA; Solvent extraction of hydrolysed OW allows the recovery of 0.8g of HT per litre. &#x025BA; Hydrolysed OW shows the highest antioxidant activity compared to raw OW.</P> <P><B>Abstract</B></P><P><I>Aspergillus niger</I> broth culture on wheat bran was assessed for olive wastewater (OW) hydrolysis in order to release hydroxytyrosol (HT). The enzyme profiles of this culture broth gave essentially (IU/L): 3000 &beta;-glucosidase and 100 esterase. Hydrolysis activity of <I>A. niger</I> enzyme preparation was evaluated by using three substrates: raw OW, phenolic fraction extracted from OW by ethyl acetate and its corresponding exhausted fraction. Large amounts of free simple phenolics were released from exhausted fraction and raw OW after enzymatic treatment. HPLC analyses show that HT was the main phenolic compound. One step of ethyl acetate extraction of hydrolysed OW allowed the recovery of 0.8g of HT per litre of OW. The antioxidant activity of extracts from OW and exhausted fraction, measured by DPPH method, was drastically enhanced after hydrolysis treatment. This study demonstrates that hydrolysed OW is a potential source of bioactive phenolic compounds with promising applications in food and pharmaceutical industries.</P>

2011

0960-8524

102

19

pp.9050-9058

10.1016/j.biortech.2011.07.048

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART57231206

Olive wastewater; Enzyme preparation; Hydrolysis; Hydroxytyrosol recovery