초록
<P><I>Schizochytrium mangrovei</I> strain PQ6 was investigated for coproduction of docosahexaenoic acid (C22: 6ω‐3, DHA) and squalene using a 30‐L bioreactor with a working volume of 15 L under various batch and fed‐batch fermentation process regimes. The fed‐batch process was a more efficient cultivation strategy for achieving higher biomass production rich in DHA and squalene. The final biomass, total lipid, unsaponifiable lipid content, and DHA productivity were 105.25 g · L<SUP>−1</SUP>, 43.40% of dry cell weight, 8.58% total lipid, and 61.66 mg · g<SUP>−1</SUP> · L<SUP>−1</SUP>, respectively, after a 96 h fed‐batch fermentation. The squalene content was highest at 48 h after feeding glucose (98.07 mg · g<SUP>−1</SUP> of lipid). Differences in lipid accumulation during fermentation were correlated with changes in ultrastructure using transmission electron microscopy and Nile Red staining of cells. The results may be of relevance to industrial‐scale coproduction of DHA and squalene in heterotrophic marine microalgae such as <I>Schizochytrium</I>.</P>