Improvement of biohydrogen production by optimization of pretreatment method and substrate to inoculum ratio from microalgal biomass and digested sludge
메타 데이터
바이오화학분류
바이오정밀화학
연료
논문
Improvement of biohydrogen production by optimization of pretreatment method and substrate to inoculum ratio from microalgal biomass and digested sludge
<P><B>Abstract</B></P> <P>Biohydrogen from microalgal biomass has shown particular advantage due to its high growth rate and high bioenergy production. As a representative of microalgae, <I>Chlorella vulgaris</I> was chosen as substrate along with digested sludge (DS) as inoculum in this research. In order to improve the hydrolysis of algal biomass and enhance biohydrogen production, pretreatment methods like acid and thermal pretreatment were employed. Thermal pretreatment showed better results than acid pretreatment of microalgal biomass. 100 °C for 60 min was identified as the optimum condition for the thermal pretreatment of <I>C. vulgaris</I> by response surface methodology (RSM) analysis. Experiments were also carried out to identify the optimum substrate to inoculum ratio (SIR) for the process. SIR of 8 generated the highest hydrogen yield of 190.90 mL H<SUB>2</SUB>/g-VS. Moreover, the overall energy balance of the process was evaluated and the results showed a positive energy balance of 1790.13 kJ/kg. The results indicated that optimization of pretreatment methods and substrate to inoculum ratio was effective in enhancing biohydrogen production from microalgal biomass and digested sludge.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Enhanced biohydrogen production using <I>C. vulgaris</I> as substrate was achieved. </LI> <LI> RSM was identified as a useful method for optimization of pretreatment condition. </LI> <LI> Optimum S/I ratio was identified using <I>C. vulgaris</I> as substrate and DS as inoculum. </LI> <LI> Overall energy balance of the process was evaluated for practical applications. </LI> </UL> </P>