BioChem - DOS

Enhanced pectinase production by optimizing fermentation conditions of Bacillus subtilis growing on hazelnut shell hydrolyzate

Journal of molecular catalysis. B, Enzymatic

Uzuner, S.; Cekmecelioglu, D.

This study describes optimization of polygalacturonase (PG) production using Bacillus subtilis in submerged fermentation by Plackett-Burman (PB) design and response surface methodology (RSM). Five variables (pH, time, temperature, yeast extract concentration and K<SUB>2</SUB>HPO<SUB>4</SUB>), which were determined to be significant by the PB analysis, were further optimized using Box-Behnken response surface method. The optimization results indicated that a maximal PG activity of 5.60UmL<SUP>-1</SUP> was achieved at pH 7.0, 72h, and 30<SUP>o</SUP>C using 0.5% (w/v) yeast extract and 0.02% (w/v) K<SUB>2</SUB>HPO<SUB>4</SUB> in the fermentation medium. The results implied a 2.7-fold increase in PG activity of B. subtilis under the optimized conditions. Thus, it was concluded that hazelnut shell hydrolyzate have remarkable potential for low cost commercial PG production.

2015

Elsevier

1381-1177

1873-3158

113

pp.62-67

10.1016/j.molcatb.2015.01.003

http://click.ndsl.kr/servlet/OpenAPIDetailView?keyValue=05787966&target=NART&cn=NART71581591

Hazelnut shell hydrolyzate; Pectinases; Plackett-Burman Design; Polygalacturonase activity; Response surface optimization